Coding

Part:BBa_M36024

Designed by: Jon Rodriguez   Group: Stanford BIOE44 - S11   (2011-05-03)

phiX174 gene A - mutated RBS's and start codons of B, K and C.

Modified PhiX174 stage III replication and packaging gene. Took the wt gene A from coliphage phiX174, and then mutated it synonymously in order to destroy the RBS's and start codons of the overlapping genes B, K, and C. Still contains the packaging tag ("Ori+") and the promoter Pb.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1402
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1402
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1402
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1402
    Illegal NgoMIV site found at 1528
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None